Mass spectrometric detection of affinity purified crosslinked presented

Gas-chromatographic Mass-spectrometric Detection

Cystathionine has been reported to undergo enzymatic changes leading to the formation of seven membered cyclic products (Ricci, G., Santoro, L., Achilli, M., Matarese, R. M., Nardini, M., and Cavallini, D. (1983) J. Biol. Chem. 258, 10511-10517; Cavallini, D., Costa, M., Pensa, B., and Coccia, R. (1985) Biochem. Int. 10, 641-646). Gas-chromatographic and mass-spectrometric evidence reported in...

An MBoC Favorite: Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes

The proteasome is a complex multifunctional machine that destroys proteins marked for ubiquitin-mediated proteolysis. In this paper, the authors employ an elegant approach to isolate and define yeast proteasomes and their suite of interacting proteins (Verma et al., 2000). This paper has something for everyone. The method the authors developed has now become the standard in the field for rapid ...

Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes.

Ubiquitin-dependent proteolysis is catalyzed by the 26S proteasome, a dynamic complex of 32 different proteins whose mode of assembly and mechanism of action are poorly understood, in part due to the difficulties encountered in purifying the intact complex. Here we describe a one-step affinity method for purifying intact 26S proteasomes, 19S regulatory caps, and 20S core particles from budding ...

Mass Spectrometric Detection of Carcinogen Adducts

Genotoxic carcinogens interact with nucleic acids and proteins to yield covalently bound products (adducts), the quantitative determination of which has been proposed as a biomonitor of human exposure to carcinogens. Mass spectrometry is playing a leading role both in the structural characterisation and in the quantitative determination of these carcinogen adducts. In particular electrospray io...

Mass Spectrometric Detection of Enzymatic Bioassays